ME1 Protein (AA 1-572) (GST tag)
Quick Overview for ME1 Protein (AA 1-572) (GST tag) (ABIN1310614)
Target
See all ME1 ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-572
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Purification tag / Conjugate
- This ME1 protein is labelled with GST tag.
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Purpose
- ME1 (Human) Recombinant Protein (P01)
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Sequence
- MEPEAPRRRH THQRGYLLTR NPHLNKDLAF TLEERQQLNI HGLLPPSFNS QEIQVLRVVK NFEHLNSDFD RYLLLMDLQD RNEKLFYRVL TSDIEKFMPI VYTPTVGLAC QQYSLVFRKP RGLFITIHDR GHIASVLNAW PEDVIKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC GGMNPQECLP VILDVGTENE ELLKDPLYIG LRQRRVRGSE YDDFLDEFME AVSSKYGMNC LIQFEDFANV NAFRLLNKYR NQYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTILFQ GAGEAALGIA HLIVMALEKE GLPKEKAIKK IWLVDSKGLI VKGRASLTQE KEKFAHEHEE MKNLEAIVQE IKPTALIGVA AIGGAFSEQI LKDMAAFNER PIIFALSNPT SKAECSAEQC YKITKGRAIF ASGSPFDPVT LPNGQTLYPG QGNNSYVFPG VALGVVACGL RQITDNIFLT TAEVIAQQVS DKHLEEGRLY PPLNTIRDVS LKIAEKIVKD AYQEKTATVY PEPQNKEAFV RSQMYSTDYD QILPDCYSWP EEVQKIQTKV DQ
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Characteristics
- Human ME1 full-length ORF ( AAH25246, 1 a.a. - 572 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)
custom-made ME1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)custom-made ME1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 226-572) protein (His tag)ME1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) protein (Myc-DYKDDDDK Tag)ME1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
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Alternative Name
- ME1
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Background
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Full Gene Name: malic enzyme 1, NADP(+)-dependent, cytosolic
Synonyms: HUMNDME,MES -
Gene ID
- 4199
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Pathways
- Regulation of Lipid Metabolism by PPARalpha
Target
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