c-MYC Protein (AA 1-439) (GST tag)

Catalog No. ABIN1311716

Product Details

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Protein Type: Recombinant

Protein Characteristics: AA 1-439

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This c-MYC protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Purpose: MYC (Human) Recombinant Protein (P01)

Sequence: MPLNVSFTNR NYDLDYDSVQ PYFYCDEEEN FYQQQQQSEL QPPAPSEDIW KKFELLPTPP LSPSRRSGLC SPSYVAVTPF SLRGDNDGGG GSFSTADQLE MVTELLGGDM VNQSFICDPD DETFIKNIII QDCMWSGFSA AAKLVSEKLA SYQAARKDSG SPNPARGHSV CSTSSLYLQD LSAAASECID PSVVFPYPLN DSSSPKSCAS QDSSAFSPSS DSLLSSTESS PQGSPEPLVL HEETPPTTSS DSEEEQEDEE EIDVVSVEKR QAPGKRSESG SPSAGGHSKP PHSPLVLKRC HVSTHQHNYA APPSTRKDYP AAKRVKLDSV RVLRQISNNR KCTSPRSSDT EENVKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSVQAEE QKLISEEDLL RKRREQLKHK LEQLRNSCA

Characteristics: Human MYC full-length ORF ( ENSP00000259523, 1 a.a. - 439 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Shoji, Suenaga, Kaneko, Islam, Alagu, Yokoi, Nio, Nakagawara: "NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells." in: Biochemical and biophysical research communications, Vol. 461, Issue 3, pp. 501-6, (2015) (PubMed).

Smith, Womack: "A matrix approach to guide IHC-based tissue biomarker development in oncology drug discovery." in: The Journal of pathology, Vol. 232, Issue 2, pp. 190-8, (2013) (PubMed).

Target Details

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: MYC (MYC Products)

Background: Full Gene Name: v-myc myelocytomatosis viral oncogene homolog (avian)
Synonyms: bHLHe39,c-Myc

Gene ID: 4609

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect