MYCN Protein (AA 1-464) (GST tag)

Catalog No. ABIN1311728

This Recombinant MYCN protein is expressed in Wheat germ and has been mentioned in 4+ publications.

Quick Overview for MYCN Protein (AA 1-464) (GST tag) (ABIN1311728)

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-464

Purification tag / Conjugate: This MYCN protein is labelled with GST tag.

Purpose: MYCN (Human) Recombinant Protein (P01)

Sequence: MPSCSTSTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW KKFELLPTPP LSPSRGFAEH SSEPPSWVTE MLLENELWGS PAEEDAFGLG GLGGLTPNPV ILQDCMWSGF SAREKLERAV SEKLQHGRGP PTAGSTAQSP GAGAASPAGR GHGGAAGAGR AGAALPAELA HPAAECVDPA VVFPFPVNKR EPAPVPAAPA SAPAAGPAVA SGAGIAAPAG APGVAPPRPG GRQTSGGDHK ALSTSGEDTL SDSDDEDDEE EDEEEEIDVV TVEKRRSSSN TKAVTTFTIT VRPKNAALGP GRAQSSELIL KRCLPIHQQH NYAAPSPYVE SEDAPPQKKI KSEASPRPLK SVIPPKAKSL SPRNSDSEDS ERRRNHNILE RQRRNDLRSS FLTLRDHVPE LVKNEKAAKV VILKKATEYV HSLQAEEHQL LLEKEKLQAR QQQLLKKIEH ARTC

Characteristics: Human MYCN full-length ORF ( NP_005369.2, 1 a.a. - 464 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Cheng, He, Chen, Cai, Gu, Pan, Duan, Wu, Wu: "P300 Interacted With N-Myc and Regulated Its Protein Stability via Altering Its Post-Translational Modifications in Neuroblastoma." in: Molecular & cellular proteomics : MCP, Vol. 22, Issue 3, pp. 100504, (2023) (PubMed).

Smith, Moreno, Heaton, Chesler, Pearson, Garrett: "Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma." in: Molecular oncology, (2015) (PubMed).

Suenaga, Islam, Alagu, Kaneko, Kato, Tanaka, Kawana, Hossain, Matsumoto, Yamamoto, Shoji, Itami, Shibata, Nakamura, Ohira, Haraguchi, Takatori, Nakagawara: "NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3? resulting in the stabilization of MYCN in human neuroblastomas." in: PLoS genetics, Vol. 10, Issue 1, pp. e1003996, (2014) (PubMed).

Smith, Womack: "A matrix approach to guide IHC-based tissue biomarker development in oncology drug discovery." in: The Journal of pathology, Vol. 232, Issue 2, pp. 190-8, (2013) (PubMed).

Target Details

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Alternative Name: MYCN

Background: Full Gene Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Synonyms: MODED,N-myc,NMYC,ODED,bHLHe37

Gene ID: 4613

NCBI Accession: NM_005378