RHOA Protein (AA 1-193) (GST tag)
Quick Overview for RHOA Protein (AA 1-193) (GST tag) (ABIN1318004)
Target
See all RHOA ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-193
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Purification tag / Conjugate
- This RHOA protein is labelled with GST tag.
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Purpose
- RHOA (Human) Recombinant Protein (P01)
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Sequence
- MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LVL
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Characteristics
- Human RHOA full-length ORF ( AAH01360, 1 a.a. - 193 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Ras Homolog Gene Family, Member A (RHOA) (AA 61-193) protein (His-IF2DI Tag)
RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) protein (His tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-191), (partial) protein (GST tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % WB, SDS, ELISA
Ras Homolog Gene Family, Member A (RHOA) protein (Myc-DYKDDDDK Tag)RHOA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- RHOA (Ras Homolog Gene Family, Member A (RHOA))
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Alternative Name
- RHOA
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Background
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Full Gene Name: ras homolog gene family, member A
Synonyms: ARH12,ARHA,RHO12,RHOH12 -
Gene ID
- 387
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Pathways
- Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome
Target
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