Melanoma gp100 Protein (AA 1-661) (GST tag)

Catalog No. ABIN1320014

This Recombinant Melanoma gp100 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for Melanoma gp100 Protein (AA 1-661) (GST tag) (ABIN1320014)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-661

Purification tag / Conjugate: This Melanoma gp100 protein is labelled with GST tag.

Purpose: SILV (Human) Recombinant Protein (P01)

Sequence: MDLVLKRCLL HLAVIGALLA VGATKVPRNQ DWLGVSRQLR TKAWNRQLYP EWTEAQRLDC WRGGQVSLKV SNDGPTLIGA NASFSIALNF PGSQKVLPDG QVIWVNNTII NGSQVWGGQP VYPQETDDAC IFPDGGPCPS GSWSQKRSFV YVWKTWGQYW QVLGGPVSGL SIGTGRAMLG THTMEVTVYH RRGSRSYVPL AHSSSAFTIT DQVPFSVSVS QLRALDGGNK HFLRNQPLTF ALQLHDPSGY LAEADLSYTW DFGDSSGTLI SRALVVTHTY LEPGPVTAQV VLQAAIPLTS CGSSPVPGTT DGHRPTAEAP NTTAGQVPTT EVVGTTPGQA PTAEPSGTTS VQVPTTEVIS TAPVQMPTAE STGMTPEKVP VSEVMGTTLA EMSTPEATGM TPAEVSIVVL SGTTAAQVTT TEWVETTARE LPIPEPEGPD ASSIMSTESI TGSLGPLLDG TATLRLVKRQ VPLDCVLYRY GSFSVTLDIV QGIESAEILQ AVPSGEGDAF ELTVSCQGGL PKEACMEISS PGCQPPAQRL CQPVLPSPAC QLVLHQILKG GSGTYCLNVS LADTNSLAVV STQLIMPGQE AGLGQVPLIV GILLVLMAVV LASLIYRRRL MKQDFSVPQL PHSSSHWLRL PRIFCSCPIG ENSPLLSGQQ V

Characteristics: Human SILV full-length ORF ( NP_008859.1, 1 a.a. - 661 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Rochin, Hurbain, Serneels, Fort, Watt, Leblanc, Marks, De Strooper, Raposo, van Niel: "BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 26, pp. 10658-63, (2013) (PubMed).

Target Details

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: SILV

Background: Full Gene Name: silver homolog (mouse)
Synonyms: D12S53E,ME20,PMEL,PMEL17,SI,SIL,gp100

Gene ID: 6490

NCBI Accession: NM_006928