SPC25 Protein (AA 1-224) (GST tag)
Quick Overview for SPC25 Protein (AA 1-224) (GST tag) (ABIN1321123)
Target
See all SPC25 ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-224
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Purification tag / Conjugate
- This SPC25 protein is labelled with GST tag.
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Purpose
- SPC25 (Human) Recombinant Protein (P01)
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Sequence
- MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYN
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Characteristics
- Human SPC25 full-length ORF ( NP_065726.1, 1 a.a. - 224 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-224) protein (His tag)
custom-made SPC25 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-226) protein (His tag)custom-made SPC25 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))
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Alternative Name
- SPC25
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Background
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Full Gene Name: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Synonyms: AD024,MGC22228,SPBC25 -
Gene ID
- 57405
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NCBI Accession
- NM_020675
Target
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