TFF3 Protein (AA 15-73) (GST tag)

Catalog No. ABIN1322573

This Recombinant TFF3 protein is expressed in Wheat germ and has been mentioned in 5+ publications.

Quick Overview for TFF3 Protein (AA 15-73) (GST tag) (ABIN1322573)

Target: TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 15-73

Purification tag / Conjugate: This TFF3 protein is labelled with GST tag.

Purpose: TFF3 (Human) Recombinant Protein (P01)

Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

Characteristics: Human TFF3 full-length ORF ( AAH17859.1, 15 a.a. - 73 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Du, Luo, Qin, Wang, Wang, Xiang, Zhang: "Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease." in: PLoS ONE, Vol. 8, Issue 11, pp. e80271, (2013) (PubMed).

Liu, Tefft, Roberts, Zhang, Ren, Li, Huang, Zhang, Phillips, Wu: "Cardioprotective proteins upregulated in the liver in response to experimental myocardial ischemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 303, Issue 12, pp. H1446-58, (2012) (PubMed).

Xue, Lü, Zhang, Wu, Huang, Wu, Sheng, Wu, Hu, Lai: "Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach." in: Journal of proteome research, Vol. 9, Issue 1, pp. 545-55, (2010) (PubMed).

Bignotti, Ravaggi, Tassi, Calza, Rossi, Falchetti, Romani, Bandiera, Odicino, Pecorelli, Santin: "Trefoil factor 3: a novel serum marker identified by gene expression profiling in high-grade endometrial carcinomas." in: British journal of cancer, Vol. 99, Issue 5, pp. 768-73, (2008) (PubMed).

Hamady, Farrar, Whitehead, Holland, Lodge, Carding: "Identification and use of the putative Bacteroides ovatus xylanase promoter for the inducible production of recombinant human proteins." in: Microbiology (Reading, England), Vol. 154, Issue Pt 10, pp. 3165-74, (2008) (PubMed).

Target Details

Target: TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

Alternative Name: TFF3

Background: Full Gene Name: trefoil factor 3 (intestinal)
Synonyms: HITF,ITF,TFI,hP1.B

Gene ID: 7033