WNT5A Protein (AA 1-380) (GST tag)

Catalog No. ABIN7596476

This Recombinant WNT5A protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for WNT5A Protein (AA 1-380) (GST tag) (ABIN7596476)

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-380

Purification tag / Conjugate: This WNT5A protein is labelled with GST tag.

Purpose: WNT5A (Human) Recombinant Protein (P01)

Sequence: MKKSIGILSP GVALGMAGSA MSSKFFLVAL AIFFSFAQVV IEANSWWSLG MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEIVDQFVCK

Characteristics: Human WNT5A full-length ORF ( NP_003383.2, 1 a.a. - 380 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Bazhin, Tambor, Dikov, Philippov, Schadendorf, Eichmüller: "cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca(2+) homeostasis in melanoma cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 5, pp. 817-28, (2010) (PubMed).

Target Details

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: WNT5A

Background: Full Gene Name: wingless-type MMTV integration site family, member 5A
Synonyms: hWNT5A

Gene ID: 7474

NCBI Accession: NM_003392

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation