IL36A/IL1F6 Protein

Catalog No. ABIN1344001

Recombinant IL36A/IL1F6 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL36A/IL1F6 Protein (ABIN1344001)

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Purpose: IL-36alpha (aa 8-160) (mouse) (rec.) (untagged)

Cross-Reactivity: Mouse

Characteristics: Mouse IL-36alpha (aa Arg8-His160) is untagged.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces secretion of IL-6 in BMDCs at a concentration of 100ng - 1μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Alternative Name: IL-36alpha

Background:

Interleukin-36alpha, Interleukin-1 Family Member 6, IL-1F6, FIL1epsilon, Interleukin-1epsilon, Interleukin-1 Homolog 1, IL-1H1

IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1 and are inhibited by IL-36Ra. The expression of IL-36 cytokines has been shown to occur at different sites including the lung and skin and can be derived from diverse cell types including keratinocytes, bronchial epithelium as well as macrophages, monocytes and different T cell subsets. IL-36 family members induce the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination. Recently a novel role for IL-36alpha in the pathogenesis of intestinal inflammation has been reported.

Molecular Weight: ~17kDa

UniProt: Q9JLA2

Pathways: Cancer Immune Checkpoints