IL1F9 Protein (DYKDDDDK Tag)
Quick Overview for IL1F9 Protein (DYKDDDDK Tag) (ABIN1344114)
Target
See all IL1F9 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This IL1F9 protein is labelled with DYKDDDDK Tag.
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Purpose
- IL-36gamma (aa 13-164) (mouse) (rec.) (FLAG)
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Cross-Reactivity
- Mouse
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Characteristics
- Mouse IL-36gamma (aa Gly13-Ser164) is fused at the C-terminus to a FLAG®-tag.
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Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
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Biological Activity Comment
- Induces secretion of IL-6 in BMDCs.
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Interleukin 1 Family Member 9 (IL1F9) (AA 13-164) protein (His tag)
IL1F9 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1 Family Member 9 (IL1F9) (AA 18-169) protein (His tag)IL1F9 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1 Family Member 9 (IL1F9) (AA 1-169) protein (His tag)IL1F9 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 100 μL sterile water.
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Concentration
- 0.1 mg/mL
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Buffer
- Contains PBS.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- IL1F9 (Interleukin 1 Family Member 9 (IL1F9))
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Alternative Name
- IL-36gamma
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Background
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Interleukin-36gamma, Interleukin-1 Family Member 9, IL-1F9
IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination.
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Molecular Weight
- ~17kDa
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UniProt
- Q8R460
Target
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