CD40 Protein (CD40) (AA 21-193) (Fc Tag)

Catalog No. ABIN1344135

This Recombinant CD40 protein is expressed in HEK-293 Cells.

Quick Overview for CD40 Protein (CD40) (AA 21-193) (Fc Tag) (ABIN1344135)

Target: CD40

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 21-193

Purification tag / Conjugate: This CD40 protein is labelled with Fc Tag.

Purpose: CD40 (human):Fc (human) (rec.)

Specificity: Binds human and mouse CD40L.

Cross-Reactivity: Human, Mouse

Characteristics: The Fc portion of human IgG1 is fused to the C-terminus of human CD40 (aa 21-193).

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Blocks the binding of CD40L to CD40.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile water. Further dilutions should be made with medium containing 5 % fetal calf serum or a carrier protein.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: CD40

Alternative Name: CD40

Background:

Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, CD40L Receptor

CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.

Molecular Weight: ~54kDa (SDS-PAGE)

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints