Clusterin Protein (CLU) (AA 23-449, Nuclear) (His tag)

Catalog No. ABIN1344142

Recombinant Clusterin protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for Clusterin Protein (CLU) (AA 23-449, Nuclear) (His tag) (ABIN1344142)

Target: Clusterin (CLU)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: Nuclear, AA 23-449

Purification tag / Conjugate: This Clusterin protein is labelled with His tag.

Purpose: Clusterin (nuclear form) (human) (rec.) (His)

Cross-Reactivity: Human

Characteristics: Human nuclear clusterin (aa 23-449) is fused at the C-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Shim, Kang, Jeon, Park, Lee, Lee, Park, Lee, Schedin, Min: "Clusterin induces matrix metalloproteinase-9 expression via ERK1/2 and PI3K/Akt/NF-?B pathways in monocytes/macrophages." in: Journal of leukocyte biology, Vol. 90, Issue 4, pp. 761-9, (2011) (PubMed).

Target Details

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background:

TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2

Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80- kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling, and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.

Molecular Weight: ~45kDa (SDS-PAGE)

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling