NUCB2 Protein (AA 25-420) (His tag)

Catalog No. ABIN1344143

This Recombinant NUCB2 protein is produced in Escherichia coli (E. coli).

Quick Overview for NUCB2 Protein (AA 25-420) (His tag) (ABIN1344143)

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 25-420

Purification tag / Conjugate: This NUCB2 protein is labelled with His tag.

Purpose: Nucleobindin-2 (mouse) (rec.)

Cross-Reactivity: Mouse

Characteristics: Mouse nucleobindin-2 (aa 25-420) is fused at the C-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.2μm-filtered solution in PBS, pH 7.2.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: Nucleobindin-2

Background:

DNA-binding Protein NEFA, Prepronesfatin, Nucb2, Nesfatin

Nucleobindin-2 (NUCB2, Prepronesfatin) was first isolated in adipocytes, but is also expressed by gastric mucosa and pancreatic beta cells. This pattern of nucleobindin-2 expression, as well as the presence of nucleobindin-2 within the plasma of rodents and humans, strongly suggests that this compound may act as a circulating regulatory factor. NUCB2 can be cleaved into three polypeptides: nesfatin-1, nesfatin-2, and nesfatin-3. Anorexigenic effects have been described for nesfatin-1 that plays an important role in hypothalamic pathways regulating food intake and energy homeostasis and acting in a leptin-independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Nucleobindin-2 is therefore reported to reduce food intake after administration, mostly refering to the cleaved peptide nesfatin-1. Excess nucleobindin-2/nesfatin-1 in the brain leads to a loss of appetite, lack of the protein in the brain leads to an increase of appetite.

Molecular Weight: ~55kDa (SDS-PAGE)

UniProt: P81117