NUCB2 Protein (AA 25-420) (His tag)
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- Target See all NUCB2 Proteins
- NUCB2 (Nucleobindin 2 (NUCB2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-420
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NUCB2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Mouse nucleobindin-2 (aa 25-420) is fused at the C-terminus to a His-tag.
- Purity
- >90 % (SDS-PAGE)
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- <1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product NUCB2 Protein
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Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)
NUCB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)NUCB2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nucleobindin 2 (NUCB2) (AA 322-420) protein (GST tag)NUCB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nucleobindin 2 (NUCB2) (AA 25-420) protein (His tag)Crystallography grade NUCB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nucleobindin 2 (NUCB2) (AA 25-106) protein (His tag)Crystallography grade NUCB2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.2μm-filtered solution in PBS, pH 7.2.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. - Expiry Date
- 6 months
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- Target
- NUCB2 (Nucleobindin 2 (NUCB2))
- Alternative Name
- NUCB2 (NUCB2 Products)
- Synonyms
- nefa Protein, MGC97715 Protein, NUCB2 Protein, nucleobindin-2 Protein, NEFA Protein, AI607786 Protein, Calnuc Protein, Nefa Protein, Nesfatin-1 Protein, p54 Protein, nucleobindin 2 Protein, nucleobindin 2 L homeolog Protein, nucb2 Protein, NUCB2 Protein, Nucb2 Protein, nucb2.L Protein
- Background
- Nucleobindin-2 (NUCB2, Prepronesfatin) was first isolated in adipocytes, but is also expressed by gastric mucosa and pancreatic beta cells. This pattern of nucleobindin-2 expression, as well as the presence of nucleobindin-2 within the plasma of rodents and humans, strongly suggests that this compound may act as a circulating regulatory factor. NUCB2 can be cleaved into three polypeptides: nesfatin-1, nesfatin-2, and nesfatin-3. Anorexigenic effects have been described for nesfatin-1 that plays an important role in hypothalamic pathways regulating food intake and energy homeostasis and acting in a leptin-independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Nucleobindin-2 is therefore reported to reduce food intake after administration, mostly refering to the cleaved peptide nesfatin-1. Excess nucleobindin-2/nesfatin-1 in the brain leads to a loss of appetite, lack of the protein in the brain leads to an increase of appetite.
- Molecular Weight
- ~55kDa (SDS-PAGE)
- UniProt
- P81117
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