Netrin 1 Protein (NTN1) (AA 25-604) (Fc Tag)

Catalog No. ABIN1344145

Recombinant Netrin 1 protein expressed in HEK-293 Cells and has been mentioned in 2+ publications.

Quick Overview for Netrin 1 Protein (NTN1) (AA 25-604) (Fc Tag) (ABIN1344145)

Target: Netrin 1 (NTN1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse, Rat

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 25-604

Purification tag / Conjugate: This Netrin 1 protein is labelled with Fc Tag.

Purpose: Netrin-1 (human):Fc (human) (rec.)

Specificity: Binds to human, mouse and rat UNC5B.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Human netrin-1 (aa 25-604) is fused at the C-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Biological Activity Comment: Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile distilled water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Rama, Goldschneider, Corset, Lambert, Pays, Mehlen: "Amyloid precursor protein regulates netrin-1-mediated commissural axon outgrowth." in: The Journal of biological chemistry, Vol. 287, Issue 35, pp. 30014-23, (2012) (PubMed).

Castets, Broutier, Molin, Brevet, Chazot, Gadot, Paquet, Mazelin, Jarrosson-Wuilleme, Scoazec, Bernet, Mehlen: "DCC constrains tumour progression via its dependence receptor activity." in: Nature, Vol. 482, Issue 7386, pp. 534-7, (2012) (PubMed).

Target Details

Target: Netrin 1 (NTN1)

Alternative Name: Netrin-1

Background:

NTN1

Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.

Molecular Weight: ~90kDa (SDS-PAGE)

UniProt: O95631

Pathways: Regulation of Cell Size