CD40 Ligand Protein (CD40LG) (AA 18-111, AA 115-260) (DYKDDDDK Tag)
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- Target See all CD40 Ligand (CD40LG) Proteins
- CD40 Ligand (CD40LG)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 115-260, AA 18-111
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Origin
- Human, Mouse, Rat
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Source
- CHO Cells
- Purification tag / Conjugate
- This CD40 Ligand protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Purpose
- CD40L (rat) (multimeric) (rec.)
- Brand
- MegaCD40L™
- Specificity
- Binds to rat, human and mouse CD40.
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
- Rat CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.02EU/μg purified protein (LAL test).
- Biological Activity Comment
- Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86).
- Top Product
- Discover our top product CD40LG Protein
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (Fc Tag)
CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag,Biotin)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 1-261) protein (His tag)custom-made CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 1-260) protein (His tag)custom-made CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) protein (Fc Tag)CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG Origin: Human, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- CD40L (CD40LG Products)
- Background
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MultimericCD40L™, ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L
MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has been shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
- Molecular Weight
- ~35-40kDa
- UniProt
- Q9Z2V2
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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