RANKL Protein (AA 157-316, Soluble) (Fc Tag)

Catalog No. ABIN1344301

Recombinant RANKL protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for RANKL Protein (AA 157-316, Soluble) (Fc Tag) (ABIN1344301)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: Soluble, AA 157-316

Purification tag / Conjugate: This RANKL protein is labelled with Fc Tag.

Purpose: Fc (human):RANKL, Soluble (mouse) (rec.)

Specificity: Binds to mouse RANK.

Cross-Reactivity: Mouse

Characteristics: Mouse RANKL (aa 157-316) is fused at the N-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces osteoclastogenesis of bone marrow-derived macrophages.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Beleut, Rajaram, Caikovski, Ayyanan, Germano, Choi, Schneider, Brisken: "Two distinct mechanisms underlie progesterone-induced proliferation in the mammary gland." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 2989-94, (2010) (PubMed).

Target Details

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: :RANKL

Background:

TRANCE, ODF, OPGL, TNFSF11, CD254

RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T cell proliferation. Important regulator of interactions between T cells and dendritic cells and may play a role in the regulation of the T cell-dependent immune response. RANKL plays an important role in the progression of Breast cancer.

Molecular Weight: ~55kDa (SDS-PAGE)

UniProt: O35235

Pathways: NF-kappaB Signaling