FTO Protein (AA 2-505) (His tag)
Quick Overview for FTO Protein (AA 2-505) (His tag) (ABIN1344349)
Target
See all FTO ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 2-505
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Purification tag / Conjugate
- This FTO protein is labelled with His tag.
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Purpose
- FTO (human) (rec.) (His)
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Cross-Reactivity
- Human
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Characteristics
- Human FTO (aa 2-505) is fused at the N-terminus to a His-tag.
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Sterility
- Sterile filtered
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Endotoxin Level
- <1EU/μg purified protein (LAL test).
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Fat Mass and Obesity-Associated (FTO) (AA 1-502) protein (His tag)
custom-made FTO Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fat Mass and Obesity-Associated (FTO) (AA 1-505) protein (His tag)custom-made FTO Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fat Mass and Obesity-Associated (FTO) (AA 1-139) protein (GST tag)FTO Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Fat Mass and Obesity-Associated (FTO) (AA 2-502) protein (His-IF2DI Tag)FTO Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Fat Mass and Obesity-Associated (FTO) protein (Myc-DYKDDDDK Tag)FTO Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.
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Handling Advice
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- FTO (Fat Mass and Obesity-Associated (FTO))
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Alternative Name
- FTO
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Background
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Fat Mass and Obesity-associated Protein, FATSO
FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse 'fused toes' mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
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Molecular Weight
- ~65kDa (SDS-PAGE)
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UniProt
- Q9C0B1
Target
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