ATP5B Protein (AA 1-529) (GST tag)

Catalog No. ABIN1346052

Recombinant ATP5B protein expressed in Wheat germ and has been mentioned in 2+ publications.

Quick Overview for ATP5B Protein (AA 1-529) (GST tag) (ABIN1346052)

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-529

Purification tag / Conjugate: This ATP5B protein is labelled with GST tag.

Purpose: ATP5B (Human) Recombinant Protein (P01)

Sequence: MLGFVGRVAA APASGALRRL TPSASLPPAQ LLLRAAPTAV HPVRDYAAQT SPSPKAGAAT GRIVAVIGAV VDVQFDEGLP PILNALEVQG RETRLVLEVA QHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARA RVALTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG TMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP LDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKI QRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGEYD HLPEQAFYMV GPIEEAVAKA DKLAEEHSS

Characteristics: Human ATP5B full-length ORF ( NP_001677.2, 1 a.a. - 529 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Hansson, Korsgren, Pontén, Korsgren: "Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins." in: The Journal of pathology, Vol. 229, Issue 5, pp. 719-28, (2013) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." in: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Target Details

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B

Background: Full Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Synonyms: ATPMB,ATPSB,MGC5231

Gene ID: 506

NCBI Accession: NM_001686

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process