CA2 Protein (AA 1-260) (GST tag)

Catalog No. ABIN1347767

This Recombinant CA2 protein is produced in Wheat germ. There is 1 publication available.

Quick Overview for CA2 Protein (AA 1-260) (GST tag) (ABIN1347767)

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-260

Purification tag / Conjugate: This CA2 protein is labelled with GST tag.

Purpose: CA2 (Human) Recombinant Protein (P01)

Sequence: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Characteristics: Human CA2 full-length ORF ( NP_000058.1, 1 a.a. - 260 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Buelli, Perico, Galbusera, Abbate, Morigi, Novelli, Gagliardini, Tentori, Rottoli, Sabadini, Saito, Kawano, Saeki, Zoja, Remuzzi, Benigni: "Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease." in: EBioMedicine, Vol. 2, Issue 5, pp. 456-66, (2015) (PubMed).

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2

Background: Full Gene Name: carbonic anhydrase II
Synonyms: CA-II,CAII,Car2

Gene ID: 760

NCBI Accession: NM_000067