CBS Protein (AA 1-551) (GST tag)

Catalog No. ABIN1348191

Recombinant CBS protein expressed in Wheat germ and has been mentioned in 4+ publications.

Quick Overview for CBS Protein (AA 1-551) (GST tag) (ABIN1348191)

Target: CBS (Cystathionine-beta-Synthase (CBS))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-551

Purification tag / Conjugate: This CBS protein is labelled with GST tag.

Purpose: CBS (Human) Recombinant Protein (P01)

Sequence: MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS RCTWQLGRPA SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG LKCELLAKCE FFNAGGSVKD RISLRMIEDA ERDGTLKPGD TIIEPTSGNT GIGLALAAAV RGYRCIIVMP EKMSSEKVDV LRALGAEIVR TPTNARFDSP ESHVGVAWRL KNEIPNSHIL DQYRNASNPL AHYDTTADEI LQQCDGKLDM LVASVGTGGT ITGIARKLKE KCPGCRIIGV DPEGSILAEP EELNQTEQTT YEVEGIGYDF IPTVLDRTVV DKWFKSNDEE AFTFARMLIA QEGLLCGGSA GSTVAVAVKA AQELQEGQRC VVILPDSVRN YMTKFLSDRW MLQKGFLKEE DLTEKKPWWW HLRVQELGLS APLTVLPTIT CGHTIEILRE KGFDQAPVVD EAGVILGMVT LGNMLSSLLA GKVQPSDQVG KVIYKQFKQI RLTDTLGRLS HILEMDHFAL VVHEQIQYHS TGKSSQRQMV FGVVTAIDLL NFVAAQERDQ K

Characteristics: Human CBS full-length ORF ( AAH11381.1, 1 a.a. - 551 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Nevoral, Žalmanová, Zámostná, Kott, Kučerová-Chrpová, Bodart, Gelaude, Procházka, Orsák, Šulc, Klein, Dvořáková, Weingartová, Víghová, Hošková, Krejčová, Jílek, Petr: "Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 51, pp. 24-35, (2015) (PubMed).

Austgen, Hermann, Dantzler, Rogers, Kline: "Hydrogen sulfide augments synaptic neurotransmission in the nucleus of the solitary tract." in: Journal of neurophysiology, Vol. 106, Issue 4, pp. 1822-32, (2011) (PubMed).

Whiteman, Li, Rose, Tan, Parkinson, Moore: "The effect of hydrogen sulfide donors on lipopolysaccharide-induced formation of inflammatory mediators in macrophages." in: Antioxidants & redox signaling, Vol. 12, Issue 10, pp. 1147-54, (2010) (PubMed).

Baskar, Li, Moore: "Hydrogen sulfide-induces DNA damage and changes in apoptotic gene expression in human lung fibroblast cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 21, Issue 1, pp. 247-55, (2006) (PubMed).

Target Details

Target: CBS (Cystathionine-beta-Synthase (CBS))

Alternative Name: CBS

Background: Full Gene Name: cystathionine-beta-synthase
Synonyms: HIP4

Gene ID: 875