EIF4E Protein (AA 1-217) (GST tag)

Catalog No. ABIN1352595

Recombinant EIF4E protein expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for EIF4E Protein (AA 1-217) (GST tag) (ABIN1352595)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-217

Purification tag / Conjugate: This EIF4E protein is labelled with GST tag.

Purpose: EIF4E (Human) Recombinant Protein (P01)

Sequence: MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLNRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

Characteristics: Human EIF4E full-length ORF ( AAH12611, 1 a.a. - 217 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Andrieu, Taieb, Baylot, Ettinger, Soubeyran, De-Thonel, Nelson, Garrido, So, Fazli, Bladou, Gleave, Iovanna, Rocchi: "Heat shock protein 27 confers resistance to androgen ablation and chemotherapy in prostate cancer cells through eIF4E." in: Oncogene, Vol. 29, Issue 13, pp. 1883-96, (2010) (PubMed).

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: Full Gene Name: eukaryotic translation initiation factor 4E
Synonyms: CBP,EIF4E1,EIF4EL1,EIF4F,MGC111573

Gene ID: 1977

Pathways: BCR Signaling