GATA3 Protein (AA 1-444) (GST tag)

Catalog No. ABIN1354834

Recombinant GATA3 protein expressed in Wheat germ and has been mentioned in 4+ publications.

Quick Overview for GATA3 Protein (AA 1-444) (GST tag) (ABIN1354834)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-444

Purification tag / Conjugate: This GATA3 protein is labelled with GST tag.

Purpose: GATA3 (Human) Recombinant Protein (P02)

Sequence: MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH SRGSMTALGG ASSSTHHPIT TYPPYVPEYS SGLFPPSSLL GGSPTGFGCK SRPKARSSTE GRECVNCGAT STPLWRRDGT GHYLCNACGL YHKMNGQNRP LIKPKRRLSA ARRAGTSCAN CQTTTTTLWR RNANGDPVCN ACGLYYKLHN INRPLTMKKE GIQTRNRKMS SKSKKCKKVH DSLEDFPKNS SFNPAALSRH MSSLSHISPF SHSSHMLTTP TPMHPPSSLS FGPHHPSSMV TAMG

Characteristics: Human GATA3 full-length ORF ( NP_001002295.1, 1 a.a. - 444 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Economou, Datta, Georgiadis, Cadot, Frenz, Maconochie: "Gata3 directly regulates early inner ear expression of Fgf10." in: Developmental biology, Vol. 374, Issue 1, pp. 210-22, (2013) (PubMed).

Hosokawa, Tanaka, Suzuki, Iwamura, Ohkubo, Endoh, Kato, Endo, Onodera, Tumes, Kanai, Sugano, Nakayama: "Functionally distinct Gata3/Chd4 complexes coordinately establish T helper 2 (Th2) cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 12, pp. 4691-6, (2013) (PubMed).

Hosokawa, Tanaka, Kato, Shinoda, Tohyama, Hanazawa, Tamaki, Hirahara, Yagi, Sakikawa, Morita, Nagira, Poyurovsky, Suzuki, Motohashi, Nakayama: "Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 46, pp. 18626-31, (2013) (PubMed).

Kuwahara, Yamashita, Shinoda, Tofukuji, Onodera, Shinnakasu, Motohashi, Hosokawa, Tumes, Iwamura, Lefebvre, Nakayama: "The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-? and suppresses T(H)2 differentiation." in: Nature immunology, Vol. 13, Issue 8, pp. 778-86, (2012) (PubMed).

Target Details

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background: Full Gene Name: GATA binding protein 3
Synonyms: HDR,MGC2346,MGC5199,MGC5445

Gene ID: 2625

NCBI Accession: NM_001002295

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation