GCH1 Protein (AA 1-250) (GST tag)

Catalog No. ABIN1354910

Product Details

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Protein Type: Recombinant

Protein Characteristics: AA 1-250

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This GCH1 protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: GCH1 (Human) Recombinant Protein (P01)

Sequence: MEKGPVRAPA EKPRGARCSN GFPERDPPRP GPSRPAEKPP RPEAKSAQPA DGWKGERPRS EEDNELNLPN LAAAYSSILS SLGENPQRQG LLKTPWRAAS AMQFFTKGYQ ETISDVLNDA IFDEDHDEMV IVKDIDMFSM CEHHLVPFVG KVHIGYLPNK QVLGLSKLAR IVEIYSRRLQ VQERLTKQIA VAITEALRPA GVGVVVEATH MCMVMRGVQK MNSKTVTSTM LGVFREDPKT REEFLTLIRS

Characteristics: Human GCH1 full-length ORF ( AAH25415.1, 1 a.a. - 250 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Widder, Chen, Li, Dikalov, Thöny, Hatakeyama, Harrison: "Regulation of tetrahydrobiopterin biosynthesis by shear stress." in: Circulation research, Vol. 101, Issue 8, pp. 830-8, (2007) (PubMed).

Target Details

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GCH1 (GCH1 Products)

Background: Full Gene Name: GTP cyclohydrolase 1
Synonyms: DYT14,DYT5,GCH,GTP-CH-1,GTPCH1

Gene ID: 2643