GLA Protein (AA 1-429) (GST tag)
(1 reference)-
- Target See all GLA Proteins
- GLA (Galactosidase, alpha (GLA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-429
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This GLA protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- GLA (Human) Recombinant Protein (P01)
- Sequence
- MQLRNPELHL GCALALRFLA LVSWDIPGAR ALDNGLARTP TMGWLHWERF MCNLDCQEEP DSCISEKLFM EMAELMVSEG WKDAGYEYLC IDDCWMAPQR DSEGRLQADP QRFPHGIRQL ANYVHSKGLK LGIYADVGNK TCAGFPGSFG YYDIDAQTFA DWGVDLLKFD GCYCDSLENL ADGYKHMSLA LNRTGRSIVY SCEWPLYMWP FQKPNYTEIR QYCNHWRNFA DIDDSWKSIK SILDWTSFNQ ERIVDVAGPG GWNDPDMLVI GNFGLSWNQQ VTQMALWAIM AAPLFMSNDL RHISPQAKAL LQDKDVIAIN QDPLGKQGYQ LRQGDNFEVW ERPLSGLAWA VAMINRQEIG GPRSYTIAVA SLGKGVACNP ACFITQLLPV KRKLGFYEWT SRLRSHINPT GTVLLQLENT MQMSLKDLL
- Characteristics
- Human GLA full-length ORF ( AAH02689, 1 a.a. - 429 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
Galactosidase, alpha (GLA) (AA 322-409) protein (His-IF2DI Tag)
GLA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Galactosidase, alpha (GLA) (Active) protein (His tag)GLA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "Integrated approach to produce a recombinant, His-tagged human ?-galactosidase A in mammalian cells." in: Biotechnology progress, Vol. 27, Issue 5, pp. 1206-17, (2011) (PubMed).
-
: "Integrated approach to produce a recombinant, His-tagged human ?-galactosidase A in mammalian cells." in: Biotechnology progress, Vol. 27, Issue 5, pp. 1206-17, (2011) (PubMed).
-
- Target
- GLA (Galactosidase, alpha (GLA))
- Alternative Name
- GLA (GLA Products)
- Background
-
Full Gene Name: galactosidase, alpha
Synonyms: GALA - Gene ID
- 2717
- Pathways
- SARS-CoV-2 Protein Interactome
-
