CDNF Protein

Catalog No. ABIN1405917

This Recombinant CDNF protein is expressed in CHO Cells.

Quick Overview for CDNF Protein (ABIN1405917)

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Product Details

Characteristics: Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)

Purification: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy.

Endotoxin Level: Less than 1EU/µg of protein as determined by LAL method

Application Details

Comment:

Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.5

Handling Advice: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Target Details

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Alternative Name: CDNF

Background: CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Molecular Weight: Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: Q49AH0