MANF Protein
Quick Overview for MANF Protein (ABIN1405920)
Target
See all MANF ProteinsProtein Type
Biological Activity
Origin
Source
Application
-
-
Characteristics
- Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke
-
Purification
-
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy. -
Endotoxin Level
- Less than 1EU/µg of protein as determined by LAL method
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Active) protein
MANF Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)MANF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) protein (GST tag)MANF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (His tag)MANF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
-
-
Comment
-
Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
-
Buffer
- PBS pH 7.4
-
Handling Advice
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
-
Storage
- -80 °C
-
-
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
-
Alternative Name
- MANF
-
Background
- MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
-
Molecular Weight
- Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
-
UniProt
- P55145
Target
-
