PDGF-AA Homodimer (Homodimer) (Active) Protein

Catalog No. ABIN1589624

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for PDGF-AA Homodimer (Homodimer) (Active) Protein (ABIN1589624)

Target: PDGF-AA Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: Homodimer

Purpose: PDGF-AA

Sequence: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Specificity: Chromosomal location:7p22

Characteristics: Length (aa):125

Endotoxin Level: < 0.1 ng per ug of PDGF-AA

Application Details

Application Notes: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: 50 mM acetic acid

Handling Advice: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Storage: RT,-20 °C,-80 °C

Storage Comment: The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: PDGF-AA Homodimer

Alternative Name: PDGF-AA

Background: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A

Molecular Weight: 28.5 kDa

Gene ID: 5154

NCBI Accession: NM_002607, NP_002598

UniProt: P04085