KIT Ligand Protein (KITLG)

Catalog No. ABIN1589652

This Recombinant KIT Ligand protein is expressed in Escherichia coli (E. coli).

Quick Overview for KIT Ligand Protein (KITLG) (ABIN1589652)

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE

Product Details

Purpose: SCF

Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Specificity: Chromosomal location:10 D1, 10 57.0 cM

Characteristics: Length (aa):165

Endotoxin Level: < 0.1 ng per μg of mSCF

Application Details

Application Notes: The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is in the range of 2-10 ng/mL.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Mouse SCF should be reconstituted in 50 mM acetic acid or water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C.

Buffer: 50 mM aceetic acid

Handling Advice: Avoid repeated freeze-thaw cycles. Freeze/thaw cycles will result in significant loss of activity.

Storage: 0 °C

Storage Comment: The lyophilized mouse SCF is best stored desiccated below 0°C. Freeze/thaw cycles will result in significant loss of activity. Avoid repeated freeze-thaw cycles.

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: SCF

Background: Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform, mouse SCF shares 93% aa sequence identity with rat SCF and 72 %-75 % with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling.
Synonyms: Kitl, Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, Kitlg, contrasted

Molecular Weight: 18.42 kDa

Gene ID: 17311

NCBI Accession: NM_013598, NP_038626

UniProt: P20826

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway