KDM5D Protein (His tag)

Catalog No. ABIN1589770

Recombinant KDM5D protein expressed in Escherichia coli (E. coli).

Quick Overview for KDM5D Protein (His tag) (ABIN1589770)

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE

Product Details

Purification tag / Conjugate: This KDM5D protein is labelled with His tag.

Purpose: Recombinant Human SMCY/JARID1D Protein

Sequence: ALLVALQRLP VRLPEGEALQ CLTERAIGWQ DRARKALASE DVTALLRQLA ELRQQLQAKP RPEEASVYTS ATACDPIREG SGNNISKVQG LLENGDSVTS PENMAPGKGS DLELLSSLLP QLTGPVLELP EAIRAPLEEL MMEGDLLEVT LDENHSIWQL LQAGQPPDLD RIRTLLELEK FEHQGSRTRS RALERRRRRQ KVDQGRNVEN LVQQELQSKR ARSSGIMSQV GREEEHYQEK ADRENMFLTP STDHSPFLKG NQNSLQHKDS GSSAACPSLM PLLQLSYSDE QQLLEHHHHH H

Specificity: Chromosomal location:Yq11, Yq11

Characteristics: Length (aa):301

Application Details

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: water,Centrifuge vial prior to opening. The lyophilized human SMCY is supplied in lyophilized form and can be reconstituted in PBS or water. This solution can be diluted into other buffered solutions or stored frozen for future use.

Buffer: 50 mM NaP, 300 mM NaCl, pH 8.0

Handling Advice: Centrifuge vial prior to opening

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized human SMCY, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Target Details

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Alternative Name: SMCY/JARID1D

Background:

Synonyms: KDM5D lysine (K)-specific demethylase 5D, HY, HYA

Description: H-Y antigen is defined as a male histocompatibility antigen that causes rejection of male skin grafts by female recipients of the same inbred strain of rodents. Male-specific, or H-Y antigen(s), are also detected by cytotoxic T cells and antibodies. H-Y antigen appears to be an integral part of the membrane of most male cells. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis. The gene (Smcy/SMCY) coding for H-Y antigen detected by T cells has been cloned. It is expressed ubiquitously in male mice and humans, and encodes an epitope that triggers a specific T-cell response in vitro. Additional epitopes coded for by different Y-chromosomal genes are probably required in vivo for the rejection of male grafts by female hosts. The molecular nature of H-Y antigen detected by antibodies on most male cells is not yet known. Testis-secreted, soluble H-Y antigen, however, was found to be identical to Müllerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates. Molecular data on this antigen or antigens are not yet available.

Molecular Weight: 33.87 kDa

Gene ID: 8284

NCBI Accession: NM_001146705, NP_001140177

UniProt: Q9BY66

Pathways: Warburg Effect