CD48 Protein (CD48) (C-Term, Extracellular Domain)

Catalog No. ABIN1684678

This Recombinant CD48 protein is produced in HEK-293T Cells.

Quick Overview for CD48 Protein (CD48) (C-Term, Extracellular Domain) (ABIN1684678)

Target: CD48

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293T Cells

Purity: > 95 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of Mouse CD48 including a C-terminal 6His tag was expressed in HEK293 cells.

Characteristics: Recombinant Mouse CD48 is a monomer protein consisting of05 amino acid residue subunits, due to glycosylation migrates as an approximately 40 kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The activity was tested by immobilized recombinant mouse CD48 (10 µg/mL) ability to bind recombinant CD2 with a linear range of 0-100 ng/mL.

Restrictions: For Research Use only

Handling

Buffer: PBS solution, pH7.2

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: CD48

Alternative Name: CD48

Background: IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT-1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T-cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

UniProt: P18181