CD80 Protein (CD80) (C-Term, Extracellular Domain)

Catalog No. ABIN1684684

This Recombinant CD80 protein is produced in HEK-293T Cells.

Quick Overview for CD80 Protein (CD80) (C-Term, Extracellular Domain) (ABIN1684684)

Target: CD80

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293T Cells

Purity: > 90 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of mouse CD80 including a C-terminal 6His tag was expressed in HEK293 cells.

Characteristics: Recombinant mouse CD80 is a monomer protein consisting of20 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The activity was tested by immobilized recombinant CD80 (1 µg/mL) ability to bind recombinant mouse CD28 in functional ELISA.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris 100 mM NaCl pH 8.0

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: CD80

Alternative Name: CD80

Background: SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and earlydifferentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints