IL-17 Protein

Catalog No. ABIN1684755

This IL-17 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-17 Protein (ABIN1684755)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %, as determined by SDS-PAGE and HPLC

Product Details

Specificity: Optimized DNA sequence encoding Mouse Interleukin-17A mature chain was expressed in Escherichia Coli.

Characteristics: Native Mouse Interleukin17A, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Mouse IL-17A is a disulfide-linked monodimeric protein consisting of 137 amino acid residue subunits, and migrates as an approximately 16kDa protein under reducing conditions in SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The activity was measured by the ability to bind Human IL17RA in a functional ELISA assay with a linear range of 10-500 ng/mL. Complete precursor sequence shown, expressed chain highlighted

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: PBS solution pH 7.5.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: IL-17

Background: IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells. IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes.Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL-33 produced a wide spectrum of cytokines and chemokines. In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

UniProt: Q62386