HSPD1 Protein (partial)

Catalog No. ABIN1686691

This Recombinant HSPD1 protein is expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for HSPD1 Protein (partial) (ABIN1686691)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Protein Type: Recombinant

Origin: Bacteria

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Functional Studies (Func)

Purity: >90%

Product Details

Protein Characteristics: partial

Sequence: EDPYEKIGAE LVKEVAKKTD DVAGDGTTTA TVLAQALVRE GLRNVAAGAN PLGLKRGIEK AVEKVTETLL KGAKEVETKE QIAATAAISA GDQSIGDLIA EAMDKVGNEG VITVEESNTF GLQLELTEGM RFDKGYISGY FVTDPERQEA VLEDPYILLV SSKVSTVKDL LPLXXXXXXX GKPLLIIAED VEGEALSTLV

Specificity: ~65 kDa

Purification: Multi-Step Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product has been certified >90% pure using SDS-PAGE analysis.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 10 mM Tris pH 8, 105 mM NaCl, 5 mM EDTA

Storage: -20 °C

References

Sun, Shen, Liu, Tan, Tian, Luo, Lai, Dai, Guo: "Heat shock protein 65 promotes atherosclerosis through impairing the properties of high density lipoprotein." in: Atherosclerosis, Vol. 237, Issue 2, pp. 853-61, (2014) (PubMed).

Adamus, Bonnah, Brown, David: "Detection of autoantibodies against heat shock proteins and collapsin response mediator proteins in autoimmune retinopathy." in: BMC ophthalmology, Vol. 13, pp. 48, (2013) (PubMed).

Target Details

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Background: HSP65 isolated from Mycobacterium bovis BCG, is a member of the HSP60 family of heat shock proteins (2, 3). HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6).

Molecular Weight: approx. 65 kDa

UniProt: Q1EHB9

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity