HOPX Protein (His tag)

Catalog No. ABIN1686718

This Recombinant HOPX protein is expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for HOPX Protein (His tag) (ABIN1686718)

Target: HOPX (HOP Homeobox (HOPX))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Functional Studies (Func)

Purity: >90%

Product Details

Purification tag / Conjugate: This HOPX protein is labelled with His tag.

Sequence: MGHHHHHHME QVNELKEKGN KALSVGNIDD ALQCYSEAIK LDPHNHVLYS NRSAAYAKKG DYQKAYEDGC KTVDLKPDWG KGYSRKAAAL EFLNRFEEAK RTYEEGLKHE ANNPQLKEGL QNMEARLAER KFMNPFNMPN LYQKLESDPR TRTLLSDPTY RELIEQLRNK PSDLGTKLQD PRIMTTLSVL LGVDLGSMDE EEEIATPPPP PPPKKETKPE PMEEDLPENK KQALKEKELG NDAYKKKDFD TALKHYDKAK ELDPTNMTYI TNQAAVYFEK GDYNKCRELC EKAIEVGREN REDYRQIAKA YARIGNSYFK EEKYKDAIHF YNKSLAEHRT PDVLKKCQQA EKILKEQERL AYINPDLALE EKNKGNECFQ KGDYPQAMKH YTEAIKRNPK DAKLYSNRAA CYTKLLEFQL ALKDCEECIQ LEPTFIKGYT RKAAALEAMK DYTKAMDVYQ KALDLDSSCK EAADGYQRCM MAQYNRHDSP EDVKRRAMAD PEVQQIMSDP AMRLILEQMQ KDPQALSEHL KNPVIAQKIQ KLMDVGLIAI R

Specificity: ~63 kDa

Characteristics: 4 µM ABIN1686717, when added to 2 µM SPR-300 (Aha1)-activated Hsp90 (2 µM, His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product has been certified >90% pure using SDS PAGE analysis. 4uM ABIN1686717, when added to 2uM SPR-300 (Aha1)-activated HSP90 (2uM, His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol

Storage: -20 °C

References

Wahyudi, Wang, McAlpine: "Dimerization of a heat shock protein 90 inhibitor enhances inhibitory activity." in: Organic & biomolecular chemistry, Vol. 12, Issue 5, pp. 765-73, (2014) (PubMed).

Willmer, Contu, Blatch, Edkins: "Knockdown of Hop downregulates RhoC expression, and decreases pseudopodia formation and migration in cancer cell lines." in: Cancer letters, Vol. 328, Issue 2, pp. 252-60, (2012) (PubMed).

Target Details

Target: HOPX (HOP Homeobox (HOPX))

Alternative Name: HOP

Background: Hop (HSP70/HSP90 Organizing Protein), or Stress-induced Phosphoprotein 1 (STI1) as it is also known, is a 60 kDa protein that belongs to the large group of co-chaperones which regulate and assist the major chaperones. It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones HSP70 and HSP90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. HSP70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the HSP70/HSP90 one (2). HOP is closely related to human 63 kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).

Molecular Weight: approx. 63 kDa

Gene ID: 10963

NCBI Accession: NP_006810

UniProt: P31948

Pathways: Regulation of Muscle Cell Differentiation