IFN alpha 2b Protein (Arg46)

Catalog No. ABIN1690785

Recombinant IFN alpha 2b protein expressed in Escherichia coli (E. coli).

Quick Overview for IFN alpha 2b Protein (Arg46) (ABIN1690785)

Target: IFN alpha 2b

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: Arg46

Purpose: Recombinant Human Interferon α2B Variant (Arg46)/IFN-α2B

Sequence: MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Characteristics: Recombinant Human Interferon-alpha2b produced in E. coli is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19,4 kDa. The Interferon-alpha2b gene was obtained from human leukocytes.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Specific Activity is greater than 1.0 x 108 IU/ mg as determined by a viral resistance assay using VSV-WISH cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IFN alpha 2b

Alternative Name: IFN-alpha 2B

Background: At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.
Alternative Names: Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2

Molecular Weight: 19.4 kDa

UniProt: P01563