BNP Protein (AA 27-134) (His tag)

Catalog No. ABIN1690896

This Recombinant BNP protein is expressed in Escherichia coli (E. coli).

Quick Overview for BNP Protein (AA 27-134) (His tag) (ABIN1690896)

Target: BNP (Brain Natriuretic Peptide (BNP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 27-134

Purification tag / Conjugate: This BNP protein is labelled with His tag.

Purpose: Recombinant Human Brain Natriuretic Peptide/BNP (His27-His134, N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH

Characteristics:

Recombinant Human Brain Natriuretic Peptide/BNP (His27-His134, N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 20 % Glycerol, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: BNP (Brain Natriuretic Peptide (BNP))

Alternative Name: Brain Natriuretic Peptide

Background: Recombinant Human Natriuretic Peptides B/NPPB is produced with our E. coli expression system. The target protein is expressed with sequence (His27-His134) of Human NPPB.
Human Natriuretic peptides B acts as a cardiac hormone, it is associated with many biological actions, such as diuresis, natriuresis, vasorelaxation, which inhibits the secretion of rennin and aldosterone. It acts as a paracrine antifibrotic factor in the heart. Natriuretic peptides B can help restore the body balance of salt and water, improves the heart function. Natriuretic peptides B binds and stimulates the cGMP production of the NPR1 receptor and binds the clearance receptor NPR3.

Molecular Weight: 14.2 kDa

UniProt: P16860

Pathways: Hormone Activity