NOL3 Protein (AA 1-208) (GST tag)

Catalog No. ABIN1690938

This Recombinant NOL3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for NOL3 Protein (AA 1-208) (GST tag) (ABIN1690938)

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-208

Purification tag / Conjugate: This NOL3 protein is labelled with GST tag.

Purpose: Recombinant Human Nucleolar Protein 3/NOL3 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGNA QERPSETIDR ERKRLVETLQ ADSGLLLDAL LARGVLTGPE YEALDALPDA ERRVRRLLLL VQGKGEAACQ ELLRCAQRTA GAPDPAWDWQ HVGPGYRDRS YDPPCPGHWT PEAPGSGTTC PGLPRASDPD EAGGPEGSEA VQSGTPEEPE PELEAEASKE AEPEPEPEPE LEPEAEAEPE PELEPEPDPE PEPDFEERDE SEDS

Characteristics:

Recombinant Human Nucleolar Protein 3/NOL3 (N-GST)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,350 mM NaCl,1 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Alternative Name: Nucleolar Protein 3/NOL3

Background: Recombinant Human Nucleolar Protein 3/NOL3 (GST-tagged) is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser208) of Human NOL3 fused with a GST tag at the N-terminus.
Nucleolar Protein 3 is encoded by NOL3 gene, multiple transcript variants encoding different isoforms have been found for this gene. So far, Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing.Isoform 2 may inhibit apoptosis.It has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53.

Molecular Weight: 48.92 kDa

UniProt: O60936