DMP1 Protein (AA 17-513) (His tag)

Catalog No. ABIN1691401

Recombinant DMP1 protein expressed in Human Cells.

Quick Overview for DMP1 Protein (AA 17-513) (His tag) (ABIN1691401)

Target: DMP1 (Dentin Matrix Acidic phosphoprotein 1 (DMP1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 17-513

Purification tag / Conjugate: This DMP1 protein is labelled with His tag.

Purpose: Recombinant Human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 (C-6His)

Sequence: LPVTRYQNNE SEDSEEWKGH LAQAPTPPLE SSESSEGSKV SSEEQANEDP SDSTQSEEGL GSDDHQYIYR LAGGFSRSTG KGGDDKDDDE DDSGDDTFGD DDSGPGPKDR QEGGNSRLGS DEDSDDTIQA SEESAPQGQD SAQDTTSESR ELDNEDRVDS KPEGGDSTQE SESEEHWVGG GSDGESSHGD GSELDDEGMQ SDDPESIRSE RGNSRMNSAG MKSKESGENS EQANTQDSGG SQLLEHPSRK IFRKSRISEE DDRSELDDNN TMEEVKSDST ENSNSRDTGL SQPRRDSKGD SQEDSKENLS QEESQNVDGP SSESSQEANL SSQENSSESQ EEVVSESRGD NPDPTTSYVE DQEDSDSSEE DSSHTLSHSK SESREEQADS ESSESLNFSE ESPESPEDEN SSSQEGLQSH SSSAESQSEE SHSEEDDSDS QDSSRSKEDS NSTESKSSSE EDGQLKNIEI ESRKLTVDAY HNKPIGDQDD NDCQDGYVDH HHHHH

Characteristics: Recombinant Human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Lys17-Tyr513) of Human DMP1 fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: DMP1 (Dentin Matrix Acidic phosphoprotein 1 (DMP1))

Alternative Name: Dentin Matrix Acidic Phosphoprotein 1

Sub Type: Fusionprotein

Background: Dentin Matrix Acidic Phosphoprotein 1 (DMP-1) is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. DMP-1 is expressed in teeth particularly in odontoblast, ameloblast, and cementoblast. DMP-1 is critical for proper mineralization of bone and dentin. DMP-1 may have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, the unphosphorylated form of DMP-1 acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase, DMP-1 is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. DMP-1 mutations have also been shown to cause rickets hypophosphatemic autosomal recessive type 1 (ARHR1).
Alternative Names: Dentin Matrix Acidic Phosphoprotein 1, DMP-1, Dentin Matrix Protein 1, DMP1

Molecular Weight: 55 kDa

UniProt: Q13316

Pathways: p53 Signaling