CD99L2 Protein (AA 26-188) (His tag)

Catalog No. ABIN1691454

This Recombinant CD99L2 protein is produced in Human Cells.

Quick Overview for CD99L2 Protein (AA 26-188) (His tag) (ABIN1691454)

Target: CD99L2 (CD99 Molecule-Like 2 (CD99L2))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 26-188

Purification tag / Conjugate: This CD99L2 protein is labelled with His tag.

Purpose: Recombinant Human CD99 Antigen-Like Protein 2/CD99L2 (C-6His)

Sequence: DFDDFNLEDA VKETSSVKQP WDHTTTTTTN RPGTTRAPAK PPGSGLDLAD ALDDQDDGRR KPGIGGRERW NHVTTTTKRP VTTRAPANTL GNDFDLADAL DDRNDRDDGR RKPIAGGGGF SDKDLEDIVG GGEYKPDKGK GDGRYGSNDD PGSGMVAEPG TIAVDHHHHH H

Characteristics:

Recombinant Human CD99 Antigen-Like Protein 2/CD99L2 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD99L2 (CD99 Molecule-Like 2 (CD99L2))

Alternative Name: CD99 Antigen-Like Protein 2/CD99L2

Background: Recombinant Human CD99 Antigen-Like Protein 2/CD99L2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp26-Ala188) of Human CD99L2 fused with a polyhistidine tag at the C-terminus.
CD99 Antigen-Like Protein 2 (CD99L2) belongs to the CD99 family. CD99L2 is a single-pass type I membrane protein and expressed in many tissues, with low expression in thymus. CD99L2 plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. CD99L2 and CD99 are involved in trans-endothelial migration of neutrophils in vitro and in the recruitment of neutrophils into inflamed peritoneum. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

Molecular Weight: 18.4 kDa

UniProt: Q8TCZ2