Clusterin Protein (CLU) (AA 23-449) (His tag,Fc Tag)

Catalog No. ABIN1691610

Recombinant Clusterin protein expressed in Human Cells and has been mentioned in 1 publication.

Quick Overview for Clusterin Protein (CLU) (AA 23-449) (His tag,Fc Tag) (ABIN1691610)

Target: Clusterin (CLU)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 23-449

Purification tag / Conjugate: This Clusterin protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Human Clusterin/ApoJ (C-Fc-6His)

Sequence: DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSG SGLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSR ASSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAK LRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQY YLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEVDD IEGRMDEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH

Characteristics: Recombinant Human Clusterin is produced by our mammalian expression system. The target protein is expressed with sequence (Asp23-Glu449) of Human CLU fused with a FC-6xHis tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

References

Son, Diamond, Volpe, Aranow, Mackay, Santiago-Schwarz: "Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression." in: Scientific reports, Vol. 7, Issue 1, pp. 270, (2017) (PubMed).

Target Details

Target: Clusterin (CLU)

Alternative Name: CLU

Sub Type: Fusionprotein

Background: Clusterin is a secreted protein which belongs to the Clusterin family. Clusterin is expressed in adult testis, heart, ovary, adrenal gland, brain and liver. Clusterin has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. In addition,Clusterin is up/ down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Alternative Names: Clusterin, Aging-Associated Gene 4 Protein, Apolipoprotein J, Apo-J, Complement Cytolysis Inhibitor, CLI, Complement-Associated Protein SP-40,40, Ku70-Binding Protein 1, NA1/NA2, Testosterone-Repressed Prostate Message 2, TRPM-2, CLU, APOJ, CLI, KUB1

Molecular Weight: 78 kDa

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling