CD300a Protein (CD300A) (AA 18-178) (His tag,Fc Tag)

Catalog No. ABIN1691642

This Recombinant CD300a protein is expressed in Human Cells.

Quick Overview for CD300a Protein (CD300A) (AA 18-178) (His tag,Fc Tag) (ABIN1691642)

Target: CD300a (CD300A)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 18-178

Purification tag / Conjugate: This CD300a protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Human Leukocyte Mono Ig-Like Receptor 1/LMIR1/CD300a (C-Fc-6His)

Sequence: LSKCRTVAGP VGGSLSVQCP YEKEHRTLNK YWCRPPQIFL CDKIVETKGS AGKRNGRVSI RDSPANLSFT VTLENLTEED AGTYWCGVDT PWLRDFHDPV VEVEVSVFPA STSMTPASIT AAKTSTITTA FPPVSSTTLF AVGATHSASI QEETEEVVNS QVDDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH

Characteristics: Recombinant Human CD300A is produced by our mammalian expression system. The target protein is expressed with sequence (Leu18-Gln178) of Human LMIR1CD300A fused with a FC-6xHis tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NACl,5 % Trehalose, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

Target Details

Target: CD300a (CD300A)

Alternative Name: LMIR1CD300A

Sub Type: Fusionprotein

Background: CD300A is a single-pass type I membrane protein which belongs to the CD300 family. It contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD300 family of myeloid immunoglobulin receptors includes activating (CD300b, CD300e) and inhibitory members (CD300a, CD300f), as well as molecules presenting a negative charge within their transmembrane domain (CD300c, CD300d). It is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. CD300A is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells.
Alternative Names: CMRF35-like molecule 8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor

Molecular Weight: 45.4 kDa

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process