GLN1 Protein (AA 2-373) (His tag)

Catalog No. ABIN1691799

This Recombinant GLN1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GLN1 Protein (AA 2-373) (His tag) (ABIN1691799)

Target: GLN1 (Glutamine Synthetase (GLN1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-373

Purification tag / Conjugate: This GLN1 protein is labelled with His tag.

Purpose: Recombinant Human Glutamine Synthetase/GLUL (C-6His)

Sequence: TTSASSHLNK GIKQVYMSLP QGEKVQAMYI WIDGTGEGLR CKTRTLDSEP KCVEELPEWN FDGSSTLQSE GSNSDMYLVP AAMFRDPFRK DPNKLVLCEV FKYNRRPAET NLRHTCKRIM DMVSNQHPWF GMEQEYTLMG TDGHPFGWPS NGFPGPQGPY YCGVGADRAY GRDIVEAHYR ACLYAGVKIA GTNAEVMPAQ WEFQIGPCEG ISMGDHLWVA RFILHRVCED FGVIATFDPK PIPGNWNGAG CHTNFSTKAM REENGLKYIE EAIEKLSKRH QYHIRAYDPK GGLDNARRLT GFHETSNIND FSAGVANRSA SIRIPRTVGQ EKKGYFEDRR PSANCDPFSV TEALIRTCLL NETGDEPFQY KNLEHHHHHH

Characteristics: Recombinant Human Glutamine Synthetase/GLUL is produced by our E. coli expression system. The target protein is expressed with sequence (Thr2-Asn373) of Human GLUL fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 50 mM Imidazole, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Sub Type: Fusionprotein

Background: Glutamine Synthetase reglutes intracellular concentration of glutamate. Glutamine Synthetase catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is an important source of energy and that takes part in cell prolifetation, inhibition of apoptosis, and cell signaling. Glutamine Synthetase is expressed during early fetal stages, and has a role in maintaining body PH by removing ammonia from circulation. Mutations in the GLUL gene are related to congenital glutamine deficiency.
Alternative Names: Glutamine Synthetase, GS, Glutamate Decarboxylase, Glutamate--Ammonia Ligase, GLUL, GLNS

Molecular Weight: 43.13 kDa

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion