NFYA Protein (AA 1-318) (GST tag)

Catalog No. ABIN1691863

This Recombinant NFYA protein is expressed in Escherichia coli (E. coli).

Quick Overview for NFYA Protein (AA 1-318) (GST tag) (ABIN1691863)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-318

Purification tag / Conjugate: This NFYA protein is labelled with GST tag.

Purpose: Recombinant Human Nuclear Transcription Factor Y Subunit α/NFYA (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM EQYTANSNSS TEQIVVQAGQ IQQQVQGQPL MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG LQQIQLVPPG QIQIQGGQAV QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ QIAVQGQQVA QTAEGQTIVY QPVNADGTIL QQVTVPVSGM ITIPAASLAG AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV PGAGSVPAIQ RIPLPGAEML EEEPLYVNAK QYNRILKRRQ ARAKLEAEGK IPKERRKYLH ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS

Characteristics:

Recombinant Human Nuclear Transcription Factor Y Subunit α/NFYA (N-GST)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: Nuclear Transcription Factor Y Subunit alpha/nfya

Background: Recombinant Human Nuclear Transcription Factor Y Subunit α/NFYA is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser318) of Human NFYA fused with a GST tag at the N-terminus.
Nuclear Transcription Factor Y Subunit α (NFYA) is a member of the NFYA/HAP2 subunit family. NFYA founctions as a heterotrimeric transcription factor , which is composed of three components, NF-YA, NF-YB and NF-YC, binds to CCAAT motifs in the promoter regions in a variety of genes. NFYA forms a highly conserved transcription factor which stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and β-actin genes.

Molecular Weight: 60.58 kDa

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha