CTCF Protein (AA 1-154)
Quick Overview for CTCF Protein (AA 1-154) (ABIN1691993)
Target
See all CTCF ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-154
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Purpose
- Recombinant Human Transcriptional Repressor CTCF/CTCF
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Sequence
- MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMI
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Characteristics
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Recombinant Human Transcriptional Repressor CTCF/CTCF
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-736) protein (His tag)
custom-made CTCF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-727) protein (His tag)custom-made CTCF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-100) protein (GST tag)CTCF Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-154) protein (His tag)CTCF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))
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Alternative Name
- Transcriptional Repressor CTCF/CTCF
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Background
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Recombinant Human Transcriptional Repressor CTCF/CTCF is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ile154) of Human CTCF.
Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelve C2H2-type zinc fingers and interacts with CHD8. CTCF is widely expressed in many tissues, and it is absent in primary spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. In addition, CTCF is also indispensable in the epigenetic regulation and chromatin remodeling. -
Molecular Weight
- 16.9 kDa
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UniProt
- P49711
Target
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