CA3 Protein (AA 2-260) (His tag)

Catalog No. ABIN1692013

Recombinant CA3 protein expressed in Escherichia coli (E. coli).

Quick Overview for CA3 Protein (AA 2-260) (His tag) (ABIN1692013)

Target: CA3 (Carbonic Anhydrase III (CA3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-260

Purification tag / Conjugate: This CA3 protein is labelled with His tag.

Purpose: Recombinant Human Carbonic Anhydrase 3/CA3 (C-6His)

Sequence: AKEWGYASHN GPDHWHELFP NAKGENQSPI ELHTKDIRHD PSLQPWSVSY DGGSAKTILN NGKTCRVVFD DTYDRSMLRG GPLPGPYRLR QFHLHWGSSD DHGSEHTVDG VKYAAELHLV HWNPKYNTFK EALKQRDGIA VIGIFLKIGH ENGEFQIFLD ALDKIKTKGK EAPFTKFDPS CLFPACRDYW TYQGSFTTPP CEECIVWLLL KEPMTVSSDQ MAKLRSLLSS AENEPPVPLV SNWRPPQPIN NRVVRASFKL EHHHHHH

Characteristics:

Recombinant Human Carbonic Anhydrase 3/CA3 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CA3 (Carbonic Anhydrase III (CA3))

Alternative Name: Carbonic Anhydrase 3/CA3

Background: Recombinant Human Carbonic Anhydrase 3/CA3 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Lys260) of Human CA3 fused with a 6His tag at the C-terminus.
Carbonic Anhydrase 3 (CA3) belongs to the α-Carbonic Anhydrase family that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and it is present at high levels in skeletal muscle with much lower levels found in cardiac and smooth muscle. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. CA3 is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.

Molecular Weight: 30.6 kDa

UniProt: P07451