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Deoxyuridine Triphosphatase (DUT) (AA 1-164) Protein

Recombinant protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN1692033

Quick Overview for Deoxyuridine Triphosphatase (DUT) (AA 1-164) Protein (ABIN1692033)

Target

See all Deoxyuridine Triphosphatase (DUT) Proteins
Deoxyuridine Triphosphatase (DUT)

Protein Type

Recombinant

Origin

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Human

Source

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Escherichia coli (E. coli)

Purity

> 95 % as determined by reducing SDS-PAGE.
  • Protein Characteristics

    AA 1-164

    Purpose

    Recombinant Human dUTP Pyrophosphatase/dUTPase

    Sequence

    MPCSEETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN

    Characteristics

    Recombinant Human Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial/DUT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn164) of Human Dutpase.

    Sterility

    0.2 μm filtered

    Endotoxin Level

    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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  • Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Buffer

    Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

    Handling Advice

    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

    Storage

    -80 °C

    Storage Comment

    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.

    Expiry Date

    6 months
  • Target

    Deoxyuridine Triphosphatase (DUT)

    Alternative Name

    Dutpase

    Target Type

    Viral Protein

    Background

    Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial (dUTPase) belongs to the dUTPase family. dUTPase exits as a homotrimer and is involved in nucleotide metabolism. dUTPase produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair.
    Alternative Names: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial, dUTPase, dUTP Pyrophosphatase, DUT

    Molecular Weight

    17.7 kDa
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