PHLDA2 Protein (AA 1-152) (His tag)
Quick Overview for PHLDA2 Protein (AA 1-152) (His tag) (ABIN1692073)
Target
See all PHLDA2 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-152
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Purification tag / Conjugate
- This PHLDA2 protein is labelled with His tag.
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Purpose
- Recombinant Human PH-Like Domain A2/PHLDA2/BWR1C/IPL/TSSC3 (C-6His)
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Sequence
- MKSPDEVLRE GELEKRSDSL FQLWKKKRGV LTSDRLSLFP ASPRARPKEL RFHSILKVDC VERTGKYVYF TIVTTDHKEI DFRCAGESCW NAAIALALID FQNRRALQDF RSRQERTAPA APAEDAVAAA AAAPSEPSEP SRPSPQPKPR TPLEHHHHHH
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Characteristics
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Recombinant Human PH-Like Domain A2/PHLDA2/BWR1C/IPL/TSSC3 (C-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2) (AA 1-144) protein (His tag)
custom-made PHLDA2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2) (AA 1-152) protein (His tag)custom-made PHLDA2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2) proteinPHLDA2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2) protein (Myc-DYKDDDDK Tag)PHLDA2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.1M NaCl, 1 mM DTT, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PHLDA2 (Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2))
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Alternative Name
- Pleckstrin Homology-Like Domain Family A Member 2/PHLDA2
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Background
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Recombinant Human Pleckstrin Homology-Like Domain Family A Member 2/PHLDA2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro152) of Human PHLDA2 fused with a 6His tag at the C-terminus.
Pleckstrin Homology-Like Domain Family A Member 2 (PHLDA2) is a peripheral membrane protein that belongs to the PHLDA2 family. PHLDA2 is expressed in the placenta and adult prostate gland. In the placenta, it is present in all cells of the villous cytotrophoblast. PHLDA2 plays a role in regulating placenta growth. PHLDA2 may act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids. -
Molecular Weight
- 18.1 kDa
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UniProt
- Q53GA4
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Pathways
- Regulation of Carbohydrate Metabolic Process
Target
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