UBE2B Protein (AA 1-152)
Quick Overview for UBE2B Protein (AA 1-152) (ABIN1692121)
Target
See all UBE2B ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-152
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Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B/HR6B
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Sequence
- GSHMSTPARR RLMRDFKRLQ EDPPVGVSGA PSENNIMQWN AVIFGPEGTP FEDGTFKLVI EFSEEYPNKP PTVRFLSKMF HPNVYADGSI CLDILQNRWS PTYDVSSILT SIQSLLDEPN PNSPANSQAA QLYQENKREY EKRVSAIVEQ SWNDS
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Characteristics
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Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B/HR6B
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (His tag)
custom-made UBE2B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (His tag)UBE2B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 1-152) protein (GST tag)UBE2B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2B (UBE2B) protein (Myc-DYKDDDDK Tag)UBE2B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 10 mM HEPES, pH 7.5.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))
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Alternative Name
- Ubiquitin-Conjugating Enzyme E2 B/UBE2B
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Target Type
- Viral Protein
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Background
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Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser152) of Human UBE2B.
Ubiquitin-Conjugating Enzyme E2 B (UBE2B) belongs to the ubiquitin-conjugating enzyme family. UBE2B can catalyze the covalent attachment of ubiquitin to other proteins, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. UBE2B is indispensability for postreplication repair of UV-damaged DNA and may be involved in neurite outgrowth.Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia. -
Molecular Weight
- 17.5 kDa
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UniProt
- P63146
Target
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