NCR3 Protein (AA 19-138) (Fc Tag)

Catalog No. ABIN1692291

This Recombinant NCR3 protein is produced in Human Cells.

Quick Overview for NCR3 Protein (AA 19-138) (Fc Tag) (ABIN1692291)

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-138

Purification tag / Conjugate: This NCR3 protein is labelled with Fc Tag.

Purpose: Recombinant Human NCR3/NKp30/CD337 (C-Fc)

Sequence: LWVSQPPEIR TLEGSSAFLP CSFNASQGRL AIGSVTWFRD EVVPGKEVRN GTPEFRGRLA PLASSRFLHD HQAELHIRDV RGHDASIYVC RVEVLGLGVG TGNGTRLVVE KEHPQLGAGT VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Characteristics:

Recombinant Human NCR3/NKp30/CD337 (C-Fc)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Alternative Name: Natural Cytotoxicity Triggering Receptor 3/NCR3

Background: Recombinant Human Natural Cytotoxicity Triggering Receptor 3/NCR3 is produced with our HEK293 expression system. The target protein is expressed with sequence (Leu19 -Thr138) of Human NCR3 fused with a FC tag at the C-terminus.
Natural Cytotoxicity Triggering Receptor 3 (NCR3) along with NKp44 and NKp46 constitute a group of receptors termed 'Natural Cytotoxicity Receptors'. They play a major role in triggering NK-mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain. NKp30 is selectively expressed both in resting and activated human NK cells. In addition, NKp30 is also involved in NK-mediated induction of dendritic cell (DC) maturation. It has been demonstrated that NK cell activation signaling specifically induces lytic activity against several tumor cell types and synthesis of new NF-κB dependent proteins during the initiation of cytotoxicity.

Molecular Weight: 40.2 kDa

UniProt: O14931

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process