Resistin Protein (RETN) (AA 19-108) (His tag)

Catalog No. ABIN1692412

This Recombinant Resistin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Resistin Protein (RETN) (AA 19-108) (His tag) (ABIN1692412)

Target: Resistin (RETN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-108

Purification tag / Conjugate: This Resistin protein is labelled with His tag.

Purpose: Recombinant Human Resistin/RETN/ADSF (C-6His)

Sequence: KTLCSMEEAI NERIQEVAGS LIFRAISSIG LECQSVTSRG DLATCPRGFA VTGCTCGSAC GSWDVRAETT CHCQCAGMDW TGARCCRVQP LEHHHHHH

Characteristics: Recombinant Human Resistin/RETN is produced with our E. coli expression system. The target protein is expressed with sequence (Lys19-Pro108) of Human RETN fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Acetic acid, pH 3.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

Target Details

Target: Resistin (RETN)

Alternative Name: RETN

Sub Type: Fusionprotein

Background: Resistin known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. The length of the resistin pre-peptide in human is 108 amino acid residues and in the mouse and rat it is 114 aa, the molecular weight is ~12.5 kDa. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus (T2DM). Resistin has been shown to cause \high levels of 'bad' cholesterol (low-density lipoprotein or LDL), increasing the risk of heart disease, resistin increases the production of LDL in human liver cells and also degrades LDL receptors in the liver. Potentially links obesity to diabetes."
Alternative Names: Resistin, Adipose tissue-specific secretory factor, Cysteine-rich secreted protein FIZZ3, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, FIZZ3, HXCP1, RSTN, RETN

Molecular Weight: 10.6 kDa

UniProt: Q9HD89

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration